单克隆抗体2A7亲和层析法纯化红细胞膜表面的CD59分子

Putihcation of CD59 from Human Etythrocyte Membrane by Affinity Chro-matography with 2A7-Sepharose 4B Column

用抗ＣＤ５９单抗２Ａ７与ＣＮＢｒ－Ｓｅｐｈａｒｏｓｅ４Ｂ偶联制备亲和层析住，从正常人红细胞膜蛋白抽提物中分离纯化出ＣＤ５９分子。本法纯化的ＣＤ５９分子量为１８～２０ＫＤａ对还原剂敏感。与ＭｃＡｂｓＭＥＭ４３和１Ｆ５有高亲和性，再掺入豚鼠红细胞后，能够抑制人补体的反应性溶血。本文还对三株抗ＣＤ５９单抗２Ａ７、ＭＥＭ４３及１Ｆ５的抗原特异性进行了初步分析。

n this study.we used the anti-CD59 McAb 2A7 coupled to CNBr-sepbarose 4B as an affinity chro-matography column,purified CD59 from human erythrocyte membrane. The Purified CD59 with a molecular mass of 18～20KDa was sensitive to DTT. After abeorbing to guinea ptg erythrocytes,the purified CD59 could render them resistantto human complement attack.The difference of antigenic specificity bewean McAbe 2A7 and MEM4 3,1F5 also havebeen discussed in this paper.