摘要
本实验利用高级液相色谱法（ＨＰＬＣ）同时测定强化乳粉中的脂溶性维生素ＶＡ、ＶＤ、ＶＥ。使用的色谱条件：μ─ＢｏｏｄｓｐａｋＣ１８柱为色谱分析柱（规格３．９×３００ｍｍ），以甲醇—水《Ｖ／Ｖ９９：１）为流动相，检测波长设置在２８０ｎｍ，样品制备采用直接用碱（４０％）进行皂化。实验测得的平均回收率ＶＡ９７．４６％，ＶＤ９８．０６％，ＶＥ１０２．４４％，变异系数分别为ＶＡ５．１３％、ＶＤ４．８８％、ＶＥ５．６９％。
In the paper,fat-soluble vitamins VA、VD、VE in milk powder were di-rected by HPLC. An μ─Bondapak C18 column(3.9×300mm,10um)being used as ana-lyst column; and mobile phase being made of MeOH-H2O(99: 1 V/V)and the UV under280nm.Sample preparation was saponificated by 40%alkali directly. The average recov-eries were repectively VA 97.46%,VD 98.06%、VE 102.44%;and variaties coeffection re-sp.were VA 5.13%、VD 4.88%、VE 5.69%.
出处
《南昌大学学报（工科版）》
CAS
1994年第2期92-97,共6页
Journal of Nanchang University(Engineering & Technology)
