影响固定化里氏木霉合成纤维素酶的动力学因素

THE INFLUENCE FACTORS ON THE CELLULASE PRODUCTION BY IMMOBILIZED TRICHODERMA REESEI CELLS

对影响固定化里氏木霉（ＴｒｉｃｈｏｄｅｒｍａｒｅｅｓｅｉＲｕｔＣ３０）细胞合成纤维素酶的主要动力学因素进行了研究。以纸浆为主要碳源，采用固定化细胞，在２５０ｍＬ摇瓶中重复分批发酵，结果表明，在一批加料条件下，适宜于固定化细胞产酶的条件是：纸浆浓度１．５％～２．０％，Ｃ／Ｎ为８，培养液的初始ｐＨ值４．０，摇瓶转速２００ｒ／ｍｉｎ及２６℃。分批添加纸浆可以减少固体底物对氧气输送和物质扩散等方面造成的不良影响，有利于增加底物的总用量，从而促进纤维素酶的合成，滤纸酶活力可达到４．０５ＩＵ／ｍＬ。

The main kinetic factors that influence the cellulase synthesis by immobilized cells of Trichoderma reesei Rut C30 have been studied in this paper.Under repeated batch fermentation in 250 mLshaking flasks using sulphate pulp as carbon source,it was found that the suitable fermentation conditions of the immobilized cells were:substrate concentration,1.5% ～2.0%;C/N,8;the initial pHvalue of culture fluid,4.0;temperature,26℃;culture flask shaking speed,200 r/min.It was alsoproved that a fed-batch fermentation could improve mass transfer and increase the total substrate concentration, and therefore could effectively promote the cellulase synthesis. When the fed-dosage of sulphate pulp was 0.4 % a day, the filter Paper activity could reach 4.05 IU/mL.