无花果的组织培养

A STUDY ON TISSUE CULTURE OF FICUS CARICA

对影响无花果外植体不定芽诱导的四个因子进行了研究：不同器官、培养基的组成、外植体的预处理和不同的品种。细胞分裂素ＢＡ的效果优于ＫＴ。茎尖和茎段不定芽诱导培养基为ｍｇ·Ｌ￣（－１）（ＭＳ＋ＢＡ），叶片的分化以浓度８ｍｇ·Ｌ￣（－１）的ＢＡ效果最好。赤霉素（ＧＡ＿３）有助于芽的萌发和生长，浓度在０．５～１ｍｇ·Ｌ￣（－１）为宜。割取嫩枝插入１／２ＭＳ，附加１ＢＡ或ＮＡＡ１．０～２．０ｍｇ·Ｌ￣（－１）的生根培养基中，根原基诱导率９５％以上，移栽成活率９０％以上。

Four factors affecting adventitious shoot formation on the explants of Ficus carica were studied.They were different organs,composition of culture medium,different cutivar and pre-treatmentof explant.The addition of 6-benzylamino-purine(BA)to the culture medium was more effectivethan the addition of 6-furfurylaminopurine(KT).Formation of adventitious on shoot tip and stemshoot were cultured with full MS+BA 1 mg.·L￣(-1).Plantlet formation on leaves occurred on MS+BA8 mg·L￣(-1).Gibbrerellic acid(0.5～1 mg·L￣(-1) can help bud germination and growth.The cuttingsof young shoot were cultured with 1/2 MS with the addition of IBA or NAA(1.0～2.0 mg·L￣(-1) ).The root-primordia induced rate will reach 95%,and the survival rate of transplantation will reach90%.