优良碱性果胶酶产生菌选育

SCREENING AND BREEDING OF PRODUCING STRAIN FOR SUPER ALKALOPHILIC PECTINOLYTIC ENZYME

通过对现有碱性果胶酶产生菌的筛选和诱变，获得了一株碱性果胶酶高产菌株Ｅｒｗｉｎｉａｃｈｒｙｓａｎｔｈｅｍｉ２０１，该菌株与出发菌相比，产酶期提前，１２ｈ的产酶量为出发菌株的４倍。试验表明，该菌株能使红麻快速脱胶，是一株很有希望用于生物制浆的优良菌株。

A variant strain Erwinia chrgsanthemi 201 with high alkalophilic pectinolytic enzymes was obtained after screening and induced mutaion. As compared with the orginal strain, the endo-polygalacturonase (endo-PG) productivity course of variant strain was more rapid, the endo-PG activity for 12 h has increased by 4 times. Experiments indicted the organism E. chrysanthemi 201 was capable of rapid alkaline retting of kenaf, a promissing strain for biopulping.