摘要
用银染技术研究了马立克氏病(MD)鸡淋巴细胞染色体上核仁形成区(NO)的变化.结果发现,MD鸡的银染NOR(Ag-NOR)数目极显著高于阴性对照鸡(P<0.01);MD鸡的Ag-NOR分布也发生变化,MD阴性对照鸡的Ag-NOR仅位于一对微小染色体上.而MD鸡的却分布于多个微小染色体以及1号和4号染色体长臂末端.
Silver staining analysis showed that the Ag-NORs of the normal control chickens were located on a pair of microchromosomes. The model number was 2, with a mean number of 1.89 ± 0.12. The Ag-NORs were found to be expanded in the chickens with March's disease, with multiple Ag-NORs in 88.2% of the metaphase cells observed. The mean number of Ag-NORs was 5.621±1.25, varied from 2 to 9 with a model number of 5. In 60.40% of metaphases, the Ag-NORs signincantly appeared on the telomeres of chromosome 1. Statistics results indicated that the Ag-NORs frequency of the chickens with Marek's disease was remarkably higher than that of the normal control chickens (P< 0.01).
出处
《南京农业大学学报》
CAS
CSCD
北大核心
1994年第1期88-92,共5页
Journal of Nanjing Agricultural University
基金
高等学校博士学科点专项科研基金
关键词
马立克氏病
鸡病
chicken
Marek's disease
nucleolar organizer regions