摘要
对β—葡聚糖酶(EC3.2.1.73)高产菌B.subtilis9126—6发酵优化的实验结果表明,最佳培养基配比是:大麦份7%,玉米粉3%,豆饼粉5%.在1L发酵罐中保持温度35—37℃、pH7.0~7.2,搅拌通风培养3d.发酵液的酶活力高达154U/ml.作者还对大麦β—葡聚糖对9126—6β—葡聚糖酶产生的诱导性机制做了初步探讨.
The barley flour is the most favorable substrate for β-glucanase production of Bacillus subtilis strain 9126-6. The medium prepared in proportion of barley flour 7%, corn meal 3% and soybean cake 5% as carbon and nitrogen sources at PH 7.0~ 7.2 for the fermentation production of β-glucanasc by B. subtiolis 9126-6 with an incubation period of 3 days at 35~37℃ under moderate agitation and aeration, the β-glucanase activity of fermentation fluid reached 154 U/ ml. The induction of barley β-glucan for β-glucanase production was also undertaken.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
1994年第1期59-64,共6页
Journal of Nanjing Agricultural University
关键词
Β-葡聚糖酶
发酵
诱导效应
β-glucan
β-glucanase
fermentation
induction induction effect