β─葡聚糖酶高产菌Bacillus　subtilis　9126—6的发酵性能研究

STUDIES ON FERMENTATION OF HIGH β-GLUCANASE PRODUCING STRAIN BACILLUS SUBTILIS 9126-6

对β—葡聚糖酶（ＥＣ３．２．１．７３）高产菌Ｂ．ｓｕｂｔｉｌｉｓ９１２６—６发酵优化的实验结果表明，最佳培养基配比是：大麦份７％，玉米粉３％，豆饼粉５％．在１Ｌ发酵罐中保持温度３５—３７℃、ｐＨ７．０～７．２，搅拌通风培养３ｄ．发酵液的酶活力高达１５４Ｕ／ｍｌ．作者还对大麦β—葡聚糖对９１２６—６β—葡聚糖酶产生的诱导性机制做了初步探讨．

The barley flour is the most favorable substrate for β-glucanase production of Bacillus subtilis strain 9126-6. The medium prepared in proportion of barley flour 7%, corn meal 3% and soybean cake 5% as carbon and nitrogen sources at PH 7.0～ 7.2 for the fermentation production of β-glucanasc by B. subtiolis 9126-6 with an incubation period of 3 days at 35～37℃ under moderate agitation and aeration, the β-glucanase activity of fermentation fluid reached 154 U/ ml. The induction of barley β-glucan for β-glucanase production was also undertaken.