冷驯化和ABA对柑桔膜稳定性的影响及膜特异蛋白质的诱导

EFFECT OF COLD ACCLIMATION AND ABA ON MEMBRANE STABILITY AND SYNTHESIS OF MEMBRANE PROTEIN IN CITRUS

冷驯化和ＡＢＡ处理均诱导兴津温州蜜柑（ｃｉｔｒｕｓｕｎｓｈｉｕＭａｒｃ）抗寒性的发育，且ＡＢＡ处理使其进程加快．在抗寒性发育过程中，叶细胞电解质外渗率、丙二醛水平下降，膜稳定性提高；过氧化物酶活性增加，出现了ｐＩ８．４新同工酶；超氧物歧化酶活性提高近１倍．并有ｐＩ５．５同工酶产生；游离态精胺、亚精胺含量增加，腐胺含量下降；细胞膜蛋白质含量增加、组分改变，４种膜特异蛋白质得以诱导，分子量分别为１０４０００，３７０００，３００００，１７０００．

Cold resistance was induced in Citrus unshiu Marc. by artificial cold acclimation(CA) and ABA, the proceeding quickened by ABA. During the development of cold resistance, the rates of cletronic conductivity and melodialdehyde contents decreased. Membrane stability became stronger. Peroxidase and surperoxidase activities, as well as free-spermine and spermidine contents incrend. PI 8.4 peroxidase isozyme and pI 5.5 surperoxidase isozyme appeared, but free-putrescine contents decreased dramatically.Membrane protein contents increased and profiles altered. Four cold specific membrane proteins (M. W.104000, 37000, 30000, 17000) were induced.