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核酸杂交法检测分娩期妇女血及新生儿尿中HCMV DNA

DETECTION OF HUMAN CYTOMEGALOVIRUS DNA WITH NUCLEIC ACID HYBRIDIZATION IN BLOOD SAMPLES 0F PUERPERANT AND URINE SAMPLES OF INFANTS
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摘要 以地高辛标记人巨细胞病毒(HCMV)DNAJ序段为探针,用核酸杂交法检测南通地区783例分娩期妇女血标本,HCMV DNA阳性者618例,感染率为78.93%;检测691例出生后1周内新生儿尿标本,HCMVDNA阳性者45例,排毒率为6.51%;其中335对母婴中HCMV DNA同时阳性者39例,垂直传播率为10.99%。本文结果证明了HCMV感染的普遍性和严重性,垂直传播是新生儿先天性HCMV感染的重要途径。 HCMV DNA in 783 blood samples from puerperants and 69l urine samples from infants were examined by nucleic acid assay with digoxigenin labelled probe. The results showed that the positive rate of HCMV infection was 78.93 percent in puerperants,and that of HCMV excretion was 6.51 percent in inrants.39 out or 355 couples of mother and their baby showed positive results.They suggested that HCMV vertical transmission might play an important role in congenital infection of neonates.
出处 《南通医学院学报》 1994年第2期151-152,共2页 ACTA Academiae Medicinae Nantong
关键词 巨细胞病毒 核酸杂交 分娩期妇女 cytomegalovirus digoxigenin probc nucleie acid hybridization puerperant neonate
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  • 1王淑凤,马静雅,张礼璧.用核酸杂交法检查北京地区幼儿园正常儿童尿中人巨细胞病毒DNA[J]病毒学报,1988(01).

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