核酸杂交法检测分娩期妇女血及新生儿尿中HCMV　DNA

DETECTION OF HUMAN CYTOMEGALOVIRUS DNA WITH NUCLEIC ACID HYBRIDIZATION IN BLOOD SAMPLES 0F PUERPERANT AND URINE SAMPLES OF INFANTS

以地高辛标记人巨细胞病毒（ＨＣＭＶ）ＤＮＡＪ序段为探针，用核酸杂交法检测南通地区７８３例分娩期妇女血标本，ＨＣＭＶ ＤＮＡ阳性者６１８例，感染率为７８．９３％；检测６９１例出生后１周内新生儿尿标本，ＨＣＭＶＤＮＡ阳性者４５例，排毒率为６．５１％；其中３３５对母婴中ＨＣＭＶ ＤＮＡ同时阳性者３９例，垂直传播率为１０．９９％。本文结果证明了ＨＣＭＶ感染的普遍性和严重性，垂直传播是新生儿先天性ＨＣＭＶ感染的重要途径。

HCMV DNA in 783 blood samples from puerperants and 69l urine samples from infants were examined by nucleic acid assay with digoxigenin labelled probe. The results showed that the positive rate of HCMV infection was 78.93 percent in puerperants,and that of HCMV excretion was 6.51 percent in inrants.39 out or 355 couples of mother and their baby showed positive results.They suggested that HCMV vertical transmission might play an important role in congenital infection of neonates.