四氧嘧啶糖尿病大鼠颌下腺颗粒曲管电镜观察和体视学分析

ELECTRON MICROSCOPY AND STEREOLOGICAL ANALYSIS OF GRANULAR CONVOLUTED TUBULES OF SUBMANDIBULAR GLANDS IN ALLOXAN DIABETIC RATS

用５％四氧嘧啶水溶液１７０ｍｇ／ｋｇ给ＳＤ大鼠皮下注射。２４小时后重复注射一次。实验组血糖值持续超过２８ｍｍｏｌ／Ｌ。两周末取颌下腺作电镜观察。实验组颗粒曲管上皮细胞呈现坏死变化。主要表现为核固缩和膜结构的破坏，多数线粒体肿胀，嵴变短、稀疏，甚至消失。与对照组相比，实验组空泡状线粒体的体积密度显著增加（Ｐ＜０．０１）。ＲＥＲ池扩张、脱颗粒，其体积密度较对照组有显著增加（Ｐ＜０．０５）。多数细胞分泌颗粒消失。它们的体积密度比对照组减少（Ｐ＜０．００１）。结果表明四氧嘧啶糖尿病大鼠颌下腺颗粒曲管上皮细胞呈显著的病理变化。

SD rats were given a subcutaneous injection of 5% alloxan(170mg/kg).Arter 24 hours they received it again. In the tested group the blood sugar level was beyond 500mg% all the time. At the end or the second week the submandlbular glands were observed with EM. Epithelial cells of granular convoluted tubules (GCT)appeared necrotic changes in the tested group.They mainly showed karyopyknosis and destruction of the membranous Structures. Most mitochondria exhibited distention and shortening,sparsity or disappearance of eristae.In comparison with the control the volume density of vacuolar mitochondria showed a significant increase in the tested group(P<0.01).The eisternac of the RER were distented and showed degranulation.The volume density of RER from the tested group showed a significant increase in comparison to that from the control group(P<0.05).The secretory granules of most cells disappeared.Their volume density was less than the control group(P<0.0l).The results showed the epithelial cells of GCT of the submandibular glands appeared obviously pathologieal changes in alloxan diabetic rats.