摘要
为进一步探索人精子鞘糖脂(hsGSL)在精卵识别中的重要作用,本文采用了Ladisch分配,Vance法,高效薄板层析及薄层扫描等方法对hsGSL进行了纯化、鉴定和定量.人精了神经节苷脂(hsGls)中脂结合唾液酸的含量为0.18±0.05μg/10~8个精子细胞;人精子中性鞘糖脂(hsNGSL)中鞘氨醇的含量为0.64±0.10μg/10~8个精子细胞.hsGls主要由GM3(26.93±8.82%),GM2(15.60±3.35%),GD3(28.94±4.02%),GDla(18.56±3.42%),及GTlb(9.87±1.56%)五种组分组成.hsNGSL主要由CMH(10.95±4.41%),CDH(16.09±2.0%),CTH-1(32.73±0.09%),CTH-2(20.95±3.05%),CQH(11.24±1.74%)及CPH(8.04±0.57%)六种组分组成.
To further explore the important role of human sperm glycosphingolipid (hsGSL) in sperm-egg recognition, human sperm gangliosides (hsGls) and neutral glycosphingolipid (hsNGSL) were purified, characterized and quantified with Ladisch and Vance method, HPTLC, and thin layer scaning. LBSA in hsGls (0.18±0.05μg/108 sperm cells) and sphingosine in hsNGSL (0.64±0.10μg/108 sperm cells)were also determined. The results showed that the hsGls composition was found to comprise mainly GM3 (26.93±8.82%), GM2(15.60±3.35%),GD3(28.94±4.02%),GD_(1a)(18.56±3.42%) and GT1b (9.87±1.56%), and the hsNGSL composition was found to comprise mainly CMH (10.95±4.41%), CDH(16.09±2.0%), CTH-1(32.73±0.09%), CTH-2(20.95±3.05%), CQH(11.24±1.74%)and CPH(8.04±0.57%).
