乳腺良、恶性病变酶活性的超微结构研究

ULTRASTRUCTURAL LOCALIZATION OF ENZYMATICAL ACTIVITIES IN THE BEMIGN AND MALIGNANT LESIONS OF

应用超微结构酶组织化学技术对乳腺良、恶性病变中３种细胞器标志酶进行定位观察。结果表明：乳腺癌组织内葡萄糖－６－磷酸酶（Ｇ－６－Ｐａｓｅ）和酸性磷酸酶（ＡＣＰａｓｅ）活性显著高于正常乳腺组织和良性病变，而碱性磷酸酶（ＡＬＰａｓｅ）活性则降低；乳腺癌间质中ＡＬＰａｓｅ活性明显增强；电镜显示：Ｇ－６－Ｐａｓｅ分布于癌细胞的内质网和核膜上，ＡＣＰａｓｅ恒定的出现于细胞核内的异染色质和核仁丝上，ＡＬＰａｓｅ分布于正常乳腺组织的肌上皮和腺上皮细胞的胞膜上。本文对上述酶活性变化的临床生物学意义进行了讨论。

Activities of ALPase,ACPase and G-6-Pase were examined and analysed in the benign and malignant lesions of the human breast. Resultsowed that there were higher activities of ACPase,G-6-Pase but with no or only slight activity of ALPase in the breast tumour cells. Differences of staining intensities ofthe 3 enzymes may indicate a good marker for differentiated diagnosis between the benign lesions and breast oarcinomas. G-6-Pase was present in the endoplasmic reticulum and nuclear membrane. ACPase was located in the areas of heterochromatin and nucleolus ALPase was found on the cell membrane of normal mammary epithelum ,and myoepithelial ,it also distributed on the nuclei of fibroblasts in the stroma of carcinoma. The biological sicnificance of the above mentioned enzymatical activities and their changes were also dircussed in this paper.