斑点免疫结合试验和酶联免疫吸附试验诊断包虫病的评价

Evaluation of DIBA & ELISA in their Diagnosis of Human Hydatidosis

用人肝棘球蚴液抗原对包虫病、非包虫病患者和健康人进行了斑点免疫结合试验（ＤＩＢＡ）和酶联免疫吸附试验（ＥＬＩＳＡ）。结果显示，这两种酶免疫检测方法的敏感性分别为９８％和１００％，特异性分别为９８．７％和９８．１％，但ＤＩＢＡ具有节省抗原、试剂，不需特殊仪器设备，结果可长期保存等优点，更适用于临床诊断和流行病学调查。

Dot-immunobinding assay(DIBA)and enzyme-linked immuno-sorbent assay(ELISA) were perfor-med with antigen from human hepatic hydatid fluid on sera of 51 patients with hydatid disease,81 patients of non-hydatid diseases and 73 healthy adults.Results showed that the sensi-tivity for the DIBA and ELISA were 98%and 100%respectively and the specificity were 98.7%and 98.1%res-pectively,t=1.02 and 0.42, P>0.05.The advantages of DIBA are more eco-nomical,simple,neednot any spe-cific instruments & equipment,andthe results could be kept for long time,thus,DIBA seems to be more suitable for clinical diagiiosis & epi-demiological survey.