摘要
以湛江叉鞭金藻为材料．用普通荧光分析法测定了ｃｈｌａ、ｃｈｉｃ的荧光发射光谱，以四乳突扁藻为材料，用普通荧光分析法和同步荧光分析法测定了ｃｈｌａ、ｃｈｌｂ的荧光发射光谱，结果表明：ｃｈｌａ、ｃｈｌｂ、ｃｈｉｃ的荧光发射峰分别为６７０ｎｍ、６５５ｎｍ、６４０ｎｍ，普通荧光分析法适于进行浮游植物所含的ｃｈｌａ、ｃｈｌｃ的定性、定量测定，而不适于进行ｃｈｌａ、ｃｈｌｂ的同时测定；同步荧光法则适于进行浮游植物中ｃｈｌａ、ｃｈｌｂ的定性、定量的同时测定。
The fluorescence spectra of extracted chlorophyll of Dicrateria zhanjianensis Hu. var. sp. and Platymonas tetratele West. were determined and studied with the common spectrofluorometric method. A newly developed synchnous spectrofluorometric method was also used in the study. The results show that the fluorescence emission wavelengths of chla, chlb and chic are 670nm, 655nm and 640nm, respectively. The common spectrofluorometric method can be used in the qualitative analysis and quantitative determination of chla and chic, but can not distinguish chla and chlb precisely. The synchnous spectrofluorometric method is suitable for qualitative and quantitative measurement of chla and chlb.
关键词
海洋
浮游植物
叶绿素
荧光分析
Common spectrofluorometric method
synchnous spectrofluorometric method
marine phytoplankton
chla
chlb
chlc
