高效液相色谱法制备甲基对硫磷农药标准品的研究

Separation and Preparation of Standard Reagent of Methyl Parathion by High Performance Liquid Chromatography

ＨＰＬＣ技术由于具有快速、灵敏、准确、分辨率高、重复性好等优点，已广泛地应用于在药分析及残留分析。但采用ＨＰＬＣ技术制各在药标准品还未见报道，首次采用反相ＨＰＬＣ技术，以Ｃ１８作固定相，用甲醇—水（７６：２４）作流动相，分离工业原油制各甲基对硫磷农药标准品，所得标准品纯度在９９％以上。并考察了流动相组成、流速、进样体积、进样量等分离甲基对硫磷原油色谱分辨率、峰形及所收集产品纯度的影响，找出了纯化甲基对硫磷样品的最佳条件。

A new method to separate and prepers methyl perathion from industry is first developed by HPLC. The purity of the prepared product is no less than 99.0%.The influences of the composition of mobile phase,the effluent volocity,the injection volume and the injection quantity on the resolution and on the purity of the product have been investigated. The optimal conditions are knowm.