骆驼蓬碱抗癌作用研究

STUDY ON ANTITUMOR EFFECT OF HARMALINE

本文采用纯的盐酸骆驼蓬碱进行了体外及体内抗癌试验。结果表明:骆驼蓬碱对Hela细胞、S—180肉瘤腹水细胞均有明显的体外细胞毒作用,半数抑制浓度IC_(50)分别为37.84μg/ml及34.7μg/ml。对艾氏腹水癌细胞的细胞毒作用较弱,IC_(50)为139.97μg/ml。在体内抑瘤试验中,骆驼蓬碱对S—180肉瘤、网状细胞肉瘤L_2、肝癌等瘤株,用22.5及45μg/kg/d两个剂量组,重复3次试验,除网状细胞肉瘤L_2有一组实验疗效不显著(P>0.05)外,其余各组都具有明显的疗效,抑瘤率为S—180 32.2—47.7％,L_2 33.2—49.6％,肝癌29.4—58.2％,均有显著差异。该药对艾氏腹水癌疗效不显著。

The antitumor effect of pure harmaline was studied in vitro and vivo.The results of experimentsshowed that harmaline has significant cytotoxicity for HeLa cells and S-180 ascite cells. The 50% inhibition concentration of cell growth (IC50) were 37.8 and 34.7 μg/ml, respectively. The cytotoxicity of harmaline for EAC cell was weaker with IC 50 of 139.97 μg/ml. In autitumor tests in vivo, harmaline possessed significant antitumor activity at the dosages of 22.5 and 45 mg/kg/d. The inhibition rate of tumor growth were 33.2 - 47.1 % for S-180, 33.2-49.6 % for L and 29.4-58.2 for hepatoma. The differences between the treated and control groups in the above experiments were significant statistically.Harmaline showed no apparent effet on EAC.