长期冻存红细胞的活性研究

A STUDY OF VIABILITY OF RFD BLOOD CELL BY LONG TERM CRYOPRESERVATION

对Ｓｕｍｉｄａ低浓度甘油速冻法及高浓度甘油缓冻法分别保存１５．５±３．３和１５．３±３．２年的红细胞活性进行了研究。结果显示，低极度甘油速冻法红细胞回收率明显高于高浓度甘油缓冻法。缓冻法红细胞盐水脆性曲线左移，脆性减低。红细胞内ＡＴＰ，２．３ＤＰＧ浓度两法间差异无显著性，但与新鲜红细胞相比，ＡＴＰ无明显变化，２．３ＤＰＧ明显减低。３１ＰＮＭＲ分析示细胞内２．３ＤＰＧ在含磷酸化合物中浓度相对减低，无机磷Ｐｉ明显升高，ｐＨ值减低。ＥＳＲ示细胞流动性与新鲜红细胞相似。表明低浓度甘油速冻法在保持细胞完整性及细胞膜脆性上优于高浓度甘油缓冻法。

The viability of red blood cell cryopreserved at -80℃ or - 196℃ state by Sumida low glycerol-rapidly cooling method (Rapid Methad) and high glycerol-slowly cooling method (Slow Method) was in vestlgated. The recovery rate of cells preserved by Rapid Method was significantly higher than that by Slow Method. The cell membrane osmatic fragility by Slow Method decreased and its fragility curve shifted to the left. Differences in ATP and 2.3 DPG concentration were not significant between two methods,but 2.3 DPG was significantly lower than that of fresh, non frozen cells.31P NMR analysis showed that the relative concentration of 2. 3 DPG decreased and Pi ircreased, pH values inside red cell decreased. ESR analysis showed no significant variation between frozen and fresh (non-frozen) red cells. The results indicated that Rapid Method is better than Slow Nethod in preserving red blood cells.