毛细管区带电泳分析阿片受体和抗独特型抗羟甲芬太尼抗体

Analysis of Opioid Receptor and Anti-Idiotypic Anti-Ohmefentanyl Antibody by Capillary Zone Electrophoresis(CZE)

应用毛细管区带电泳（ＣＺＥ）分析测定阿片受体、抗独特型抗羟甲芬太尼抗体等生物大分子样品。熔融石英毛细管柱为６０ｃｍ×１００μｍｉ．ｄ．（从进样到检测器长度为５０ｃｍ），以硼砂－氢氧化钾为缓冲液。结果表明缓冲液的ｐＨ影响ＣＺＥ对阿片受体的分析，当ｐＨ为９．０时分析效果最理想，重现性良好，测得的两个主峰与同批样品进行ＳＤＳ－ＰＡＧＥ分析得到的两个条带相符。用ＣＺＥ分析抗独特型抗羟甲芬太尼抗体（ＩｇＧ），表明用ＰｒＯｔｅｉｎＡ－Ｓｅｐｈａｒｏｓｅ亲和色谱分离得到部分纯化的ＩｇＧ，迁移时间不同于对照豚鼠的ＩｇＧ。

ome biological macromolecules e.g. opioid receptor and anti-idiotypic anti-ohmefentanyl antibody were analysed by CZE on untreated silica capillary 100μm i.d.× 60cm(50cm to detector)with 25mmol/L borate buffer(pH 9.10).The results showed that the pH of buffer influenced the separation of opioid receptor.Good separation and reproducibility were obtained in the buffer of pH 9.0.Two main peaks were obtained in the separation of this receptor. The results coincided with SDS-PAGE which exhibited two bands in the plate. The analysis of anti-idiotypic anti-ohmefentanyl antibody by CZE indicated that the sample was puri-fied partially by Protein A-Sepharose affinity chromatography and distinguished from unimmunized guinea pig antibody.