植物单糖的高效液相色谱分析

High Performance Liquid Chromatographic Analvsis of Plant Monosaccharides

植物单糖的高效液相色谱分析吴伟志，罗廉，余世袁（南京林业大学理化中心南京２１００３７）１前言植物单糖（葡萄糖、木糖、半乳糖、阿拉伯糖、甘露糖等）是组成植物纤维素、半纤维素的主要单体。常规的菲林法、二硝基水杨酸法等化学分析方法￣［１］只能测定总还原糖，...

onosaccharides, including glucose,xylose,galactose,arabinose and mannose,are structure units ofplant cellulose and hemicellulose, Optimal separation of them was achieved by a Bio- Rad Aminex HPX-87Pcolumn at 85℃ eluted with degassed water at 0.6mL/min. Sample pretreatment was perfOrmed with lead ac-etate or lead nitrate, and no sugar Ioss was observed.Quantitative analysis was conducted with n- pentanol asthe internal standard Or with external standard method.Experiments over a year suggested that this methodcould provide a simple and accurate analysis for monosaccharide;present in pulping waste liquor, lignocellu-losic hydrolysates and plant extractives.