高效液相色谱测定人血清中鬼臼乙叉甙

Analysis of Etoposide in Human Serum by High Performance Liquid Chromatography(HPLC)

高效液相色谱测定人血清中鬼臼乙叉甙朱光文，刘会臣，李新芳，沈丽霞，郭巧兰，高慧钦（白求恩国际和平医院药理室石家庄０５００８２）（河北医学院药学系石家庄０５００１７）１前言鬼臼乙叉甙（Ｅｔｏｐｏｓｉｄｅ，ＶＰ－１６）是临床治疗呼吸道恶性肿瘤和白血病的较...

A rapid and simple HPLC method was developed for the quantitative analysis of etopo-side in human serum. After being extracted with ethyl acetate, etoposide was analyzed by re-versed-phase HPLC column(YWG-C_(18)H_(37)) and UV-detector(230nm).The mobile phase wasamixture of water-acetonitrile-glacial acetic acid(64: 35 :1),with a flow rate of 1mL/min. Over the concentration range of 0. 085～85μmol/L, the linear regression equation was:Y=292.17X+29.50(n=8,γ=0. 9902).The average recoveries for within day and betweenday measurements were 98.1%(n=5,CV=3.6%)and 100.5%(n=5,CV=3.9%)re-spectively.