氨基苯磺酸和氨基萘磺酸在反相离子对色谱和离子色谱中分离行为的比较

Comparison of Retention Behaviors of Phenylamine and Naphthyl-amine Sulphonic Acids in Reversed-Phase Ion-pair Liquid Chromato-graphy(RP-IPC) with in Ion Exchange Chromatography(IEC)

本文考察了氨基苯磺酸和氨基萘磺酸染料中间体在反相离子对色谱和离子色谱中的分离行为，结果表明一些异构体在反相色谱中的保留顺序与离子色谱中的顺序发生颠倒。静电作用力在离子色谱中对保留值的贡献比在反相离子对色谱中对保留值的贡献要大，而疏水作用力在反相离子对色谱中对保留值的贡献却较在离子色谱中对保留值的贡献要大。离子对色谱更有利于有机离子的分离分析。

he capacity factors of phenylamine and naphthylamine sulphonic acids in RP-IPC and IEC were measured. It has been observed that the retention order of some phenylamine and naphthylamine sulphonic acidsin RP-IPC is altered in IEC. The electrostatic interaction plays a more important role in the retention of sul-phonic acids in IEC than one does in RP-IPC and there is a stronger effect of salt concentration on the reten-tion in IEC than that in RP-IPC,However,the hydrophobic interaction plays a more important role in reten-tion of sulphonic acids in RP-IPC than it does in IEC. From our experimental findings,the RP-IPC is moresuitable for the separation of phenylamine and naphthylamine sulphonic acids.