高效凝胶渗透色谱法研究超氧化物歧化酶的分子量

Studies on the Molecular Weight of Superoxide Dismutase(SOD )by Gel Permeation Chromatography(GPC)

本文采用凝胶渗透色谱仪，对修饰后的牛血ＳＯＤ进行测定。仪器：美国ＷａｔｅｒｓＡＬＣ／ＧＰＣ２４４型高效液相色谱仪，ＳｈｏｄｅｘＰ－８２葡聚精为高分子标样。采用紫外和示差折光双检测器进行测定。所得数据与经典的凝胶电泳法完全一致，为此类高分子材料的Ｍｎ、ＭＷ和Ｄ值的测定，建立了准确、快速的测定方法。

n the paper,the molecular weights and their distribution of Cu, Zn-SOD obtained from cow blood hasbeen determined by GPC by using μBondagel E-300 and E500 series columns,water with 0.02%NaN_3 aselucnt , RI and UV detectors and puliulan P-82 as standard The experiment can be accomplished in sevenminutes, The results are the saine as by gel electrophoresis.