高效液相色谱法测定银杏叶提取物中槲皮素的含量

Determinatlon of Quercetin in Extract of Ginkgo biloba L. Leaves by High Performance Liquid Chromatography(HPLC )

高效液相色谱法测定银杏叶提取物中槲皮素的含量张晓琦，陈颖怡（陕西省微生物研究所西安７１００４３）１前言槲皮素是银杏叶提取物中黄酮类化合物的主要成分之一￣［１］。由于银杏叶中黄酮类化合物对治疗冠心病、心绞痛、高血压、支气管哮喘等有显著效果，国内外相继有...

n HPLC method has been developed for the determination of quercetin,one of flavones,in extract ofGinkgo b1loba L,leaves.Quercetin was separated completely on a Zorbax-ODS column, 250mm× 4. 6mm i.d.,using a mixture of methanol, water and phosphoric acid(55:44. 6:0.4)as mobile phase; with UV detec-tion at 254nm. The results have shown that the recoveries were between 93.3～103.3%andCVwas l.4l%。