高效液相色谱法同时测定饲料中黄曲霉毒素B_1与杂色曲霉素

Studies on Simultaneous Determination of Anatoxin B_1 andsterigma-tocystin in Feed by High Performance Liquid Chromatography(HPLC)

高效液相色谱法同时测定饲料中黄曲霉毒素Ｂ＿１与杂色曲霉素黄化成，赵尊行李寅宾（山东农业大学泰安２７１０１８）（山东省进出口商品检验局青岛２６６００２）１前言黄曲霉毒素Ｂ＿１（ＡｆｌａｔｏｘｉｎＢ＿１，简写ＡＦ）是最毒的肝毒素之一，杂色曲霉素（Ｓｔｅｒ...

his paper describes a method for simultaneous determination of aflatoxin B_1 and sterigmatocystin in feed byHPLC,The methed used μ Bondapak C_(18) column,tetrahydrofuran:methanol : water(5:1:4 mobile phase andfluorescence detector at λ_(cx)=3 6 5nm andλ_(cm)=43 5nm. The sample was extracted with acetonitrile and 40%sul-furic acid containing 4%potassium chloride(9:1).After filtration and defatting, water was added.It was fur-ther extracted with chloroform and then evaporated in a rotary evaporator.The residue was dissolved in the mo-bile phase for analysis,The minimun detectable amounts were 0.05ng and 0.2ng,and the recoveries were90.6%and 92.0%for aflatoxin B_1 and sterigmatocystin respectively.