银杏叶提取物中黄酮类成分的高效液相色谱分析

Analysis and Determination of the Flavonoids from Ginkgo Biloba Extract by High Performance Liquid Chromatography

银杏叶提取物中黄酮类成分的高效液相色谱分析谢大年，郭兆贵（湖南医科大学，临床药理国家培训中心长沙４１００７８）１前言银杏叶提取物（ＥｘｔｒａｃｔＧｉｎｋｇｏｂｉｌｏｂａ，ＥＧｂ）作为治疗应用已有几个世纪，在德国和法国已成为医生最常开的处方药品之一。我...

uantitative reversed-phase high performance liquid chromatographic method has been developed for theseparation and determination of the flavonoids found in the extracts of the leaves of ginkgo biloba L. (EGb).Procedure includes hydrolysis of the flavonoids and subsequent quantitative chromatographic assay of the ob-tained aglycones on a C_(18) column with methanol-0.4% orthophosphoric acid (55:45) as eluent. The detec-tion was effected at 360nm, A good linear relationship of standard(quercetin)was found in the range of 0.001 and 0.2μg. The coefficients of variation of standard and sample(EGb) were 2.2%and 3.1%, respec-tively, Four batches of EGb were tested by the mentioned method. The total content of ginkgo flavone glyco-sides was 23.4%(W/W).