高效液相色谱法测定超滤后血清中的假尿苷

The Determination of Pseudouridine in Serum by High Performance Liquid Chromatography(HPLC)

用高效液相色谱法（ＨＰＬＣ）直接测定了超滤后血清中的假尿苷（ＰＳＥ），平均回收率为９７．７８％，在０．０９３～６μｍｏｌ范围内呈线性关系（γ＝０．９９５６），急性白血病患者血中ＰＳＥ水平显著高于正常对照组（Ｐ＜０．０１）。该方法简单、方便、迅速，可用于恶性病的病情及疗效监测。

seudouridine(PSE)in sera,ultrafiltrated,was directly determined by HPLC with average recovery of97.78%,The relationship between peak area and amount of PSE was positively related(γ=0.9956).Thereis remarkable distinction on the amount of PSE between the patient of leukemia and normal person.Themethod is rapid, simple and convenient. It can be used to evaluate the state of malignant disease and the ef-fect of the treatment on this kind of disease.