端羟基硅胶与环状酸酐反应制备分离蛋白质的高效弱阳离子交换色谱填料

The Synthesis of Packings of Weak Cation Exchange Chromatography for Protein Separations by Reaction of Cycloanhydride to Silica with End Hydroxy Group

硅胶与含环氧基有机硅烷反应、产物与二元醇加成后再与环状酸酐反应，制得分离蛋白质的弱阳离子交换色谱填料。

he synthesis and chromatographic characteristics of four kinds of packings of high performance weakcation exchange chromatography for protein separations have been studied.Silica at first reacted with γ- glyci-doxypropyltrimethoxy silane. Then a diol compound was added for further reaction in the presence of a cata-lyst.The product reacted with a cycloanhydride and a packing for high performance weak cation exchangechromatography was obtained. It is showed that different kinds of diol and anhydride affect obviously the re-tention and resolution of proteins,and the effect of the anhydride is greater than that of the diol.The bioactive recovery of protein was 89%. The packings can be used in the separation of proteins and enzymes.