摘要
从长期受工业污水污染的河水、污泥中分离到４７株能以丙烯腈或丙腈为唯一碳、氮源或氮源生长的菌株．经高压液相色谱（ＨＰＬＣ）测试发现有５株菌在以０．１５ｍｏｌ／Ｌ丙烯腈为底物的转化反应中，产物除丙烯酸外几乎无副产物．探索了菌株６－１３的基本培养特性及其酶促反应条件．在选定条件下．通过分批补加丙烯腈，连续反应４ｈ，反应液中累积丙烯酸０．５２６ｍｏｌ／Ｌ，未检出丙烯酰胺，丙烯腈转化率高于９６％，丙烯酸由红外光谱和质谱确证．
Production of acrylic acid by the microbe cells containing nitrilase has beeninvestigated.A number of strains utilizing acrylonitrile of propionitrile as sole carbonand nitrogen source were isolated from industrial effluent and activated sludge,five ofthem could convert acrylonitrile to actylic acid with few by-products measured byHPLC.The basic culture and reaction conditions for acrylic acid production with strain6-13 were studied.Under selected conditions,0.526mol/L of acrylic acid wasaccumulated in the reaction solution through a continual reaction for 4h. The conversionrate of acrylonitrile was 96.3%.Acrylic acid was identified by IR spectrum and Massspectrum.
出处
《山东大学学报（自然科学版）》
CSCD
1994年第2期217-223,共7页
Journal of Shandong University(Natural Science Edition)
关键词
丙烯酸
微生物转化
丙烯腈
acrylic acid
microbial conversion
acrylonitrile
