摘要
在兔延髓中缝隐核(NRO)区用玻璃微电极引导单位放电383个,放电较均匀,多数幅度中等,部分放电高大,约半数的放电频率较低,均频5.4±0.3Hz,其中8.1%(31个)的放电与呼吸节律一致。针刺“人中”穴和将兔窒息分别使86%(42/49)和54.1%(53/98)的放电出现反应,针刺“人中”穴和使兔窒息均在部分紧张性单位放电诱发出呼吸节律。电刺激脑桥臂旁内侧核(NPBM)使57.6%(49/85)的单位放电出现反应,且低频放电主要表现兴奋。表明NRO内含有一定量的呼吸相关神经元,针刺“人中”穴可部分通过改变NRO神经元的活动发挥作用,NRO与NPBM之间可能存在较直接的联系。*P<0.001电均匀而稀疏,吸气性放电不呈递增型,与吸气前运动神经元放电形式不同;(3)对窒息较敏感,呈兴奋性反应。延髓内的呼吸相关神经元主要集中在背侧和腹侧呼吸组,但在其他区域也呈散在分布[5]。NRO内的呼吸相关神经元数量不多,不呈集中分布之势。许多实验表明,针刺“人中”穴对呼吸有重要影响[6],而针刺效应与延髓中缝核群的活动有着密切的关系[7]。我们观察到针刺“人中”穴可使呼吸兴奋,呼吸频幅均增。针刺“人中”穴使大多数NRO?
Experiments were performed on rabbits which were urethane anesthetized, vagatomized, gallamine paralyzed and artificially ventilated. Totally 383 unit discharges were recorded extracellulary from nucleus raphe obscurus ( NRO ) area with glass microelectrode. Nearly half of the units discharges at low frequency ( 5. 4 ± 0. 3Hz ) . 8. 1 % ( 31/383 ) of the units showed respiratory rhythm. 86 % ( 42 / 49 ) units were responsive to acupuncturing the 'Renzhong' point and 54.1% ( 53/98 ) units responsed to asphyxia. Respiratory rhythm could be induced in some tonic discharges by both acupuncturing the 'Renzhong' point and asphyxia. 57. 6 % ( 49/85 )units were responsive to stimulation of NPBM ( nucleus parabrachial medialis), and the response of the low-firing-rate units was mainly excitatory.These results suggest that NRO have some respiratory-related neurons.Effects of acupuncturing the' Renzhong' point may achieved partly through changing the activities of the NRO neurons and that there are neural connections between NRO and NPBM.
出处
《山东医科大学学报》
1994年第2期105-109,共5页
Acta Academiae Medicinae Shandong
关键词
中缝核
单位放电
电刺激
窒息
人中
Raphe nuclei
Microelectrodes
Unit discharge
Electric stimulation
Asphyxia
Renzhong
Rabbits