Ⅱ．大鼠1－烯丙基氯－3亚急性中毒神经组织cAMP、cGMP及CaM含量变化

Ⅱ.3-CHLOROPROPENE-1 EFFECTS ON CONTENT OF cAMP,cGMP AND CaM IN RAT NERVE TISSUES

为探讨１－烯丙基氯－３的神经毒作用机理，用Ｗｉｓｔａｒ大鼠形成动物疾病模型，用放免法（ＲＩＡ）及酶联免疫吸附法（ＥＬＩＳＡ）分别测定了大鼠１－烯丙基氯－３亚急性中毒后神经组织中ｃＡＭＰ、ｃＧＭＰ和游离钙调素（ＣａＭ）的含量。结果表明，实验组大鼠纹状体、小脑和坐骨腓神经中ｃＡＭＰ含量增加，ｃＧＭＰ和游离ＣａＭ则低于对照组。这一指标的变化程度与染毒剂量及组织有关。

chloropropene-1 is a kind of neurotoxicant. In this study.Wistar rats were used as 3-chloropropene- 1 intoxication test model.cAMP and cGMP level in rat nerve tissues were determined by RIA and CaM by ELISA.The result showed that cAMP content in striatum, cerebellum and sciatic-peroneal nerve of the 3-chloropropene-1 treated rats were increased,while cGMP and CaM content in the above mentioned tissues were decreased correspondingly. For cAMP, cGMP and CaM are closely related with Ca￣(2+). It is sugge ted that the destruction of Ca￣(2+) homestasis may account for 3-chlorpropene-1 neurotoxicity.