1－烯丙基氯－3对神经细胞线粒体琥珀酸脱氢酶活性的影响

THE EFFECT OF SUCCINODEHYDROGENASE ACTIVITY IN NERVE CELL MITOCHONDRIA INDUCED BY 3-CHLOROPROPENE-1

选择ＮＧ１０８－１５细胞系，用ＭＴＴ方法研究１－烯丙基氯－３对神经细胞线粒体琥珀酸脱氢酶活性的影响。结果表明，琥珀酸脱氢酶活性随毒物浓度的增加而降低，呈明显的剂量－反应关系；琥珀酸脱氢酶的活性与毒物的作用时间有关，与接触１ｈ相比，接触２４ｈ后酶的活性明显降低，毒性明显增强。ＩＣ＿（５０）接触１ｈ为１８６．５３ｍｍｏｌ／Ｌ，接触２４ｈ为４．０５ｍｍｏｌ／Ｌ，毒性增加４６倍。提示１－烯丙基氯－３对神经细胞线粒体功能有明显的抑制作用。

NG108-15 cell line and MTT method were used to research the activity of mitochondria succinodehydrogenase induced by 3-chloropropene-1.The results showed that the activity of succinodehydrogenase decreased with the increased toxic concentrations, and indicated a markble doserespones relationship;compared with 1 hr exposure,the activity of succinodehydrogenase exposed 24 hr fell sharply, the cytotoxicity increased apparently; the inhibitive concentration (IC_(50)) of 1 hr exposure is 185.53mmol/L, of 24 hr exposure 4.05mmol/L, the toxicity increased about 46 times.The effect can show that 3-chloropropene-1 can inhibit the mitochondria function of nerve cell.