LaCl_3、PrCl_3对叶绿体光合磷酸化偶联因子活性影响的研究

INFLUENCE OF LaCl_3 AND PrCl_3 ON PHOTOPHOSPHORYLATION COUPLED FACTOR ACTIVE

用０．１５ｍｍｏｌ／ＬＬａＣ１３、ＰｒＣ１３对提取的菠菜叶绿体处理后，测定了Ｃａ＃一ＡＴＰ酶、Ｍｇ＃－ＡＴＰ酶活性。结果表明，ＬａＣ１３处理后的叶绿体Ｍｇ＃－ＡＴＰ酶、Ｃａ＃－ＡＴＰ酶活力均高于对照。其相对活力分别为１０５．３，１５１．４；ＰＣＩ１３处理后的叶绿体Ｃａ＃－ＡＴＰ酶、Ｍｇ＃－ＡＴＰ酶相对活力分别为１３２．３、１３５．０。说明，ＬａＣ１３对Ｃａ＃－ＡＴＰ酶活性具有较大的激活作用，而ＰｒＣ１３对于Ｃａ３＃、Ｍｇ＃－ＡＴＰ酶活性均具有较大的激活作用。２ｍｍｏｌ／ＬＬａＣ１３．ＰｒＣ１３处理的叶片（真空渗入法）照先２０分钟后，无机磷含量均明显低于对照。说明ＬａＣ１３、ＰｒＣ１３对于菠菜光合磷酸化具有明显的促进作用。这种作用是对ＣＦ１因子激活而产生。

The experimtent measured Ca -ATPase and Mg-ATPase activities after the extracted spinach chloroplasts were treated by 0.15mmol/L LaC13, and PrCI13.The result showed that Ca-ATPase and Mg-ATPase activities are higher than the contrast.With LaC13 treating cholroplasts,the relative activities are 105.3 and 151.4 separately:with PrC13,relative activities are 132.3 and 135.0.It indicated that LaC13,has acceleration on Ca-ATPase activity,andprC13, has acceleration on both Ca-ATPase and Mg-ATPase.After leave treated by LaC13,and PrC13,were illuminated with light,content of inorganic phosphorus got low,the result showed that LaC13 and PrC13 are obvious proviede with accelerating action on photophosphorytation of the spenach chloroplasts.