苹果叶片愈伤组织诱导及其再分化植株的研究

Studies on Callus Induction of Apple Leaves and Its Re─differentiation Plants

将刻伤的苹果叶片置床于ＬＳ＋ＢＡ５ｍｇ／Ｌ＋２，４－Ｄ０．１ｍｇ／Ｌ培养基上，诱导产生愈伤组织，再将愈伤组织接种在ＬＳ＋ＢＡ５ｍｇ／Ｌ＋２，４－Ｄ０．０５ｍｇ／Ｌ培养基上，在２５℃黑暗条件下培养２周至２个月，得到再分化产生的不定芽。培养不定芽长到足够高度（２～３ｃｍ）时，切取芽苗，经发根处理液处理，插入发根床，３周至１个月后，得到具有根系的完全植株。

Apple Leaves with incised wound were bedded in the medium of LS+BA5mg/L+ 2,4-D 0. 1mg/L,and some callus was emerged by inducing on it. Then,it was inoculated on the medium of LS+BA5mg/L+ 2, 4─D 0. 05mg/L,and was cultured at 25℃ for two weeks to two momths under the dark condition. Some adventitious buds were redifferentiated around the cutting points. The bud was cut down when it was 2～3cm high,and was treated with rooting promoter (Liquid),and then was inserted in a rooting bed. A complete tooted plan was obtained after three weeds to one month.