胃和结肠癌组织中u－PA受体表达的病理意义

Pathologic Significance of Determination of u-Plasminogen Activator Receptors in Stomach and Carcinoma

用尿型纤溶酶原活化素（ｕ－ＰＡ）单抗（ＭｏＡｂ），以抗配体抗体ＬｓＡＢ免疫组化法，检测了３５例胃癌和１９例结肠癌组织中尿型纤溶酶原活化素受体（ｕ－ＰＡＲ）。结果表明，胃和结肠癌组织中ｕ－ＰＡＲ阳性表达率分别为２８．５７％和３６．８４％，两者间无明显差异（Ｐ＞０．０５）。浸润型肿瘤组织的ｕ－ＰＭ阳性表达率显著高于膨胀型（Ｐ＜０．０５），且ｕ－ＰＡＲ阳性表达多见于癌组织中散在的肿瘤细胞表面，成团或巢状生长的肿瘤细胞表面表达则较少。同时检测同一组织标本癌基因蛋白Ｃ－ｅｒｂＢ－２，经图像分析ｕ－ＰＡＲ和Ｃ－ｅｒｂＢ－２表达间具有显著相关性（ｒ＝０．８０２８，Ｐ＜０．０５）。提示ｕ－ＰＡＲ阳性表达与肿瘤细胞浸润能力增强有关。

Urokinase-type plasminogen activator receptor (u-PAR) was determined in 35 cases of stomach carcinoma and 19 cases of colon carcinoma by using anti-ligand immunohistochemical method with u-PA MoAb. Results showed that the positive rate of u-PAR in stomach and colon carcinoma were 28.75% and 36.84% respectively, and no significant difference was found between them (P>0.05) .In 54 specimens of GI cancer, the positive rate of u-PAR in case of infiltrative type was significantly higher than that of the expanding type (P<0.05),and the intense expression of u-PAR was frequently observed in cancer cell which grew dispersedly .Meanwhile,the oncoprotein C-erbB-2 was also examined in the same specimens .By aids of image cytometry,the correlation between the expression of u-PAR and C-erbB-2 was analyzed showing high correlativity of 0.8028 (P<0.05).The results indicated that u-PAR over expression was relevant to the increase of infiltrative ability of cancer cells.