中国汉族人HLA纯合细胞及狨猴细胞系DP基因结构分析

THE HLA-DPBI GENE STRUCTURES OF CHINESE HAN HOMOZYGOUS CELLS AND A MARMOSET CELL LINE

应用聚合酶链反应、基因克隆和末端终止技术，测定了分属ＤＲ９、ＤＲ１１和ＤＲ１２的６个中国汉族人ＨＬＡ纯合细胞ＤＰＢ１基因第二外显子的核苷酸序列。结果表明，６个纯合细胞分别带有４种等位特异性ＤＰＢ１＊０２０１２、ＤＰＢ１＊０２０２、ＤＰＢ１＊０５０１、ＤＰＢ１＊１３０１，基因结构和白种人相同，因而采用国外参考试剂和探针对中国人标本作ＤＰＢ１基因分型是可行的。同时，首次揭示了狨猴ＤＰＢ１基因第二外显子序列，该序列和人类ＤＰ基因同源性为６９．９％。

By Sanger's chain termination method, the HLA-DPB1 gene second exons of six Chinese HAN consanquineous homozygous cell lines were sequenced. Except that a single strand DNA clone of SMY-23 cell showed a changed code for the 40th amino acid as a somatic mutation,no'new'allele was discovered,indicating a good homology between Chinese and other ethnic groups' DNA samples in terms of nucleotide sequences at least for the four DPB1 alleles, DPB1*02012, DPB1*0202, DPB1*0501and DPB1*1301. In this article. studies on Saoe-DPB1*0101, a marmoset DP allele detected by sequencing the POR amplified segment of cell line B95-8 with the same primers for the HLA-DPB1 second exons was reported for the first time. The SaoeDPB1*0101 shared 58 amino acids with the HLA-DPB1 prototype 0101 in 83 amino acids of first doman of DP beta chain(69. 9%),which revealed an intimate evolutionary relationship between DPB1 genes of human and marmoset Sanguinus oedipus.