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检测类风湿因子ELISA法的建立及其应用 被引量:3

MEASUREMENT OF RHEUMATOID FACTORS BY ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) AND ITS APPLICATION
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摘要 本文建立了测定类风湿因子(RF)总量的双抗原夹心法和测定IgG-RF的间接法。用于临床检测表明,100名献血员中有3例RF总量测定为阳性。97例类风关患者中72例阳性(阳性率75.3%)。132例健康老年人中5人阳性(阳性率3.8%)。IgG-RF测定结果:120例正常人和132例健康老年人均为阴性,97例类风关病人中有7例阳性(阳性率7.2%)。 Two ELISA methods for detection of RFs have been developed in this laboratory: a double antigen sandwich ELISA and an indirect ELISA. The double antigop.sandwich ELISA was applied to quantitation of total RFs. The test was reproducible with intra-and inter-assay coefficients of 7. 3410 and 8. 86%, respectively. The sensitivity of this assay was 5IU/ml. Sera from 100 blood donors, 97RA patients and 132 healthy retired workers were tested by this method. In thegroup of blood donors RF levels were above normal level (10IU/ml) in 3 casesonly. SeromoSitive rate of RF in RA patients was 75. 2%: in healthy retired workers (>50 years old) was 3.8%.The indirect ELISA was applied specifically to the detection of IgG-RF. Sera from 120 donors and 132 healthy retired workers were tested, none of them showed detectable IgG-RF. In 97 RA patients, 7 cases were positive.
出处 《上海免疫学杂志》 CSCD 北大核心 1994年第3期173-175,共3页 Shanghai Journal of Immunology
关键词 类风湿因子 IgG-RF ELISA Rheumatoid Factor(RF),ELISA, IgG-RF
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  • 1王加银,冯根宝,武建国.双抗原夹心ELISA法检测类风湿因子[J]临床检验杂志,1986(01).

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