摘要
本研究是在研制成ＥＤＳ７６油乳灭活苗的基础上．用高压匀浆机代替原先使用的高速均装机来大批量生产疫苗．系统地研究了整个工艺流程中质量控制的各种因素，确定了水相和油相比例、乳化剂和稳定剂的ＨＬＢ值和乳化压力这３个技术参数．结果表明３３％的水相、复合乳化剂的ＨＬＢ值为７．１和在２０Ｐ压力下初次乳化。再在３０Ｐ压力下匀乳的工艺流程能获得稳定性好、粘度低、质量可靠的ＥＤＳ７６油乳剂灭活疫苗，适宜于生产实际使用．
on the successful preparing EDS-76 oil-emulsion vaccine in the laboratory,the high-Pres sure homogenizer replaced the high-speed mixer to use for mass-producing the vaccine in this study.In order to control the-vaccine quality,the technology proccss had been studied through determing three parameters: the water-phase preportion the HLB value of compound emulsifier and the emulsifier and the emulsification pressures.The results showed that produced vaccine with 33 percent of water-phase proportion,7.1 HLB value of the compound emusifier, and the first emulsification under 20 P,again emulsification under 30 P,has good Stability and low viscosity,and is applicable to poultry industry.
关键词
鸡病
减蛋综合症
疫苗
灭活疫苗
油乳剂
EDS-76
watr-proportion
HLB value
Emulsification pressure
