石刁柏的花药培养与植株再生

ANTHER CULTURE AND PLANT REGENERATION OF Asparagus officinalis

报道了石刁柏不同品种以及不同时期采集的花药在不同培养基上的培养效果。结果表明：１）品种间愈伤组织诱导率差异明显（其中以Ｍ·Ｗ５００最高，达３０．５％，ＵＣ１５７最低，仅７．０％）；２）适宜的花药采集期为５月中、下旬；３）在ＮＡＡ：ＢＡ为１∶１配比的培养基上能获１１．０％的再生植株和１４．８％的绿芽；４）已分化的绿芽转入１／２ＭＳ基本培养基附加０．１ｍｇ／ＬＮＡＡ的培养基有助于根的分化和生长。

The anthers from different asparagus varieties and various collection dates were cultured on solidified MS medium supplemented with 2,4-D 1 mg/L, NAA 0. 2 mg/L,6-BA 0. 5mg/L and 3% sucrose to induce callus. Optimum anther culture period was May,the callus induction rate was 38. 7%. The callus induction rates of anthers from varieties M. W500, UC 309, UC72, UC711 and UC 157 were 30. 5%, 27. 5%, 18. 7%, 17. 4%, and 6. 9%, respectively. The calluses were transferred to various differential media, MS medium supplemented with NAA 1mg/L and 6-BA 1 mg/L was most efficient, 20% of calluses formed shoot apexes or plantlets. When those shoot apexes were transferred to MS basal medium and subcultured for 20～40 days, 32. 5% of shoots rooted.