摘要
猪轮状病毒对传代细胞的适应性研究结果表明，病毒须用胰蛋白酶（２５μｇ／ｍｌ）置３７℃处理１ｈ，病毒才具有对细胞的感染性，每次病毒传代须经此法处理，病毒才能连续传代。病毒传代过程中，细胞培养的维持液须不含牛血清。ＬＬＣ－ＭＫ＿２猴肾传代细胞能适应猪轮状病毒培养。
Study on the cell culture of porcine rotavirus was conducted. The virus possesses the infectivity to cell after being treated for 1 hour at 37℃ by trypsin (25μg/ml). The porcine rotavirus can adapt to LLC-MK cell line. Serum must be absent in maintenance liquid of cell culture.
出处
《上海农业学报》
CSCD
1994年第3期10-13,共4页
Acta Agriculturae Shanghai
