β－内啡肽在小鼠脾淋巴细胞转化过程中的调节作用

REGULATORY EFFECT OF β-ENDORPHIN ON SPLENOCYTE PROLIFERATION IN MICE

本文采用淋巴细胞转化（淋转）试验法观察到β－内啡肽在浓度为０．３，３，３０ｎｍｏｌ／Ｌ时，可增强亚适量刀豆蛋白Ａ（ＣｏｎＡ）（０．６２５ｍｇ／Ｌ）诱导的小鼠脾淋转反应，而对未经活化的静止淋巴细胞未见致分裂作用。作者又观察到纳洛酮作为阿片受体的拮抗剂，可阻断β－内啡肽对小鼠脾淋转反应的增强作用。另外，发现在ＣｏｎＡ诱导淋转过程中不同时间加入β－内啡肽，其协同效应从培养起始后２４ｈ内最为明显。

Using lymphocyte transformation test as an indioator,we investigated the regulatory effect of β-endorphin on splenocyte proliferation in mice.The results showed that β-endorphin in doses of 0.3,3,30 nmol/L enhanced the proliferation response of splenocytes to Con A in a suboptimal concentration(0.625 mg/L)but did not have any effect on resting,unstimulated spleen cells.The maximal effect of β-endorphin on lymphocyte proliferation was seen when it was added to the experimental system within 24 hours after the commencement of culture.The effect of β-endorphin is reversed by naloxone,an opiate peptide antagonist.