外源性神经节苷脂对人肝癌培养细胞生长、蛋白分泌及合成的影响

EFFECTS OF EXOGENOUS GM_3 AND GD_3 ON CELL GROWTH,PROTEIN SECRETION AND SYNTHESIS OF HUMAN HEPATOCARCINOMA CELL LINE

１０ｍｇ／Ｌ双唾液酸神经节苷脂（ＧＤ３）可促进人肝癌ＳＭＭＣ－７７２１细胞生长，增加细胞ＤＮＡ合成，静止期细胞数减少而合成期和分裂期细胞增多。单唾液酸神经节苷脂（ＧＭ３）对白蛋白的分泌和合成均发生抑制，而ＧＤ３对白蛋白的分泌也发生抑制，但细胞内白蛋白的含量上升。ＧＭ３、ＧＤ３对甲胎蛋白的合成影响不大，ＧＤ３可降低甲胎蛋白的分泌，而ＧＭ３对甲胎蛋白的分泌影响不大。结果提示ＧＤ３有促进细胞生长作用，ＧＭ３、ＧＤ３比对不同蛋白质的分泌和合成有选择性作用。

At the concentration of 10mg/L, exogenous GD3 could stimulate cell growth of human hepatocartcinoma cell line SMMC-7721, which was most apparent at the fourth and fifth day of cell circle. It was proved through the method of 3H-dT incorporation and cell analysis system that GD3 could increase DNA synthesis and stimulate cell transition from G0/G1 phase to S/M phase.GM3 (10mg/L) could inhibit albumin (ALB) secretion and synthesis; GD3 (10mg/L) could also inhibit ALB secretion, but increase the ALB percentage of cellular proteins. GM3/GD3 had slightly effects on AFP synthesis. GD3 could decrease AFP secretion, but GM3 had no obvious effects on AFP secretion. These results suggested that GD3 could stimulate cell growth, and GM3/GD3 had selective effects on secretion and synthesis of different proteins.