PCR检测DYZ－1基因在产前性别鉴定中的应用──附44例羊水检测报告

DETECTING DYZ-1 GENE FOR DETERMINING FETAL SEX BY POLYMERASE CHAIN REACTION

作者采用多聚酶链反应（ＰｏｌｙｍｅｒａｓｅＣｈａｉｎＲｅａｃｔｉｏｎＰＣＲ）技术检测染色体长臂上的ＤＹＺ－１基因，用４４例羊水进行了产前胎儿性别鉴定，其中２３例证实为男性，２１例为女性，同时用常规羊水细胞培养进行染色体核型分析，二者结果符合率达１００％，结果表明ＰＣＲ技术用于产前胎儿性别鉴定具有特异性强、灵敏度高、正确率高、方法简捷等优点。

olymerase chain reaction(PCR)assay was used to amplify DYZ-1 gene on the Ychromosome in 44 amniotic fluid cells for determining fetal sex. 2 3 fetuses were foundto be males and 2 1 fetuses were found to be females.Meanwhile the karyotypes of 2m- niotic cells were analyzed by the conventional cytogenetic methods with a correct identi- fication rate of 1 00%. The results show that using PCR technique for determiningfetal sex is reliable,sensitive,rapid and simple.