胎脑细胞悬液的制备及活力测定

PREPARATION AND CELL VIABILITY DETERMINATION OE FETAL BRAIN CELL SUSPENSION

水囊引产胎儿７例，取其大脑皮层和中脑腹侧组织分别应用胰酶消化法和研磨过滤法制备胎脑细胞悬液，应用吖啶橙—溴化乙锭荧光染色来判定悬液中细胞活力，结果表明：应用胰酶消化法制备的大脑皮层和中脑腹侧胎脑细胞悬液中的细胞活力分别显著地高于应用研磨过滤法制备的大脑皮层和中脑腹侧胎脑细胞悬液（ｔ检验，Ｐ＜０．００１）；应用两种方法制备的大脑皮层胎脑细胞悬液的活力分别显著地高于两种方法制备的中脑腹侧胎脑细胞悬液（Ｐ＜０．００１）。本文讨论了胎儿胎龄、所取部位和以上两种制备方法对细胞活力的影响。研究表明吖啶橙一嗅化乙锭荧光染色可作为体外判定细胞活力快速而有效的方法，应用以上两种方法制备的胎脑细胞悬液在常温下放置５～６小时内仍可被用于脑内移植。

he cerebral cortex and ventral mesencephalon tissue from seven induced labor em-bryoes are made into a fetal brain cell suspension by trypsin incubation or grind-fiItra-tion. Acridine orange and ethidium bromide fluorepent stain is used to determine thecell viability. The results show that the cell viability of both cerebral corteX and ven-tral mesencephalon cell suspension prepared by trypsin incubation are significantly high-er than that by grind-filtration(t’test,p<0.001).The cell viability of the cerebralcorteX suspension by both above methods are significantly higher than that in the ven-tral mesencephalon suspension(p<0.001).The paper discribes tbat the cell viability isaf fected by embryo age,brain cell location and the both above methods of preparation.The research results demonstrate that the embryonic cell suspension prepared by the bothabove methods after stored at normal atmospheric temperature in vitro for 5 to 6 hours,may still be used to intracerebral transpl antation.