大鼠颈上神经节脑内移植治疗帕金森氏病的实验研究

TRANSPLANTATION OF THE SUPERIOR CERVICAL GANGLION TO THE ADULT RAT BRAIN FOR EXPERIMENTAL THERAPY OF PARKINSON DISEASE

用６－羟基多巴损毁大鼠黑质建立拟Ｐａｒｋｉｎｓｏｎ氏病模型。黑质重度损毁１５只和部分损毁１３只，随机取前者１２只，后者１０只进行同种成年颈上神经节向尾状核内移植，其余６只移植坐骨神经片段作为对照。移植前后用ａｐｏｍｏｒｐｈｉｎｅ诱发旋转观察其行为改变。重度损毁组移植后，旋转速率减少１０％～８０％者占５８．３３％，部分损毁组移植后旋转速率减少１０％～１００％者占８０％，对照组旋转行为无变化。移植１～２个月后，用乙醛酸诱发荧光组化方法和尼氏染色观察旋转次数明显减少的动物，发现其端脑切片均有存活的移植神经元和大量儿茶酚胺特异荧光膨体纤维，并有时可见膨体荧光纤维伸入宿主尾状核内。而旋转未减少的鼠脑中，移植物存活很少。本文对颈上神经节脑内移植治疗作用的机理进行了讨论并为临床应用提供了重要资料。

As an experimental model , the adult Sprague-Dawley rats in which the dopamine system in substaintia nigra (SN) had been unilaterally destroyed by 6-hydroxydopamine (6-OHDA) were chosen. Twenty eight rats were divided into two groups group of severe lesion (SL): 15 rats. group of moderate lesion (ML): 13 rats. Twelve in the former group and ten in the latter group received homograft of mature superior cervical ganglion (SCG) to the caudate nucleus ipsilateral to the 6-OHDA lesion. The remaining six were transplanted with pieces of sciatic nerve used for controls. The rats were tested for rotation in response to apomorphine both before and after grafting. The probability of turning speed was reduced by 58. 33% after graft (from) 10% to 80%) in SL group, and the probability of turning speed was reduced by 80% (from 10% to 100% ) after graft in ML group. Rotational behaviour did not change in control animals. At sacrifice, l～2 months after transplantation.the brain tissue treated by the glyoxylic acid fluorescence histochemical and Nissl stain method was observed. In the rats in which rotational behaviour was significantly reduced we found surviving neurons of the graft and numerous varicose fibers with strongly specific green fluorescence indicating the presence of catecholamine. while the rats in which rotatory response did not change had very few surviving neurons in grafts. This paper discusses the mechanisms for the amelioration of 6-OH-DA-induced parkinsonism by SCG transplantation in the rat brain. This result provides valuable data for clinical therapy.