人胎儿腹腔神经节的细胞分化──荧光组化法观察

THE OBSERVATION OF CELL DIFFERENTIATION IN THE COELIAC GANGLIA OF HUMAN EMBRYOS WITH HISTOCHEMICAL FLUORESCENCE METHOD

本文以乙醛酸诱发儿茶酚胺荧光法检查了２０例胎龄１４～４０周的人胎儿、早产儿和新生儿腹腔神经节。结果显示在胎龄约１４～１６周时，腹腔节细胞由密集成群并显强烈儿茶酚胺荧光的前体细胞构成；随着胎龄增大，节内这些前体细胞逐渐被弱荧光、体积较大的主神经元取代。至出生前，绝大部细胞已为主神经元，仅存少量小强荧光细胞。小强荧光细胞的分布形式除有成体交感神经节中所见到的单个散在和小团外，尚有由较多小强荧光细胞集聚并包以结缔组织被膜的小强荧光细胞小节。早产儿和新生儿腹腔节的小强荧光细胞的胞体大小、形态以及荧光强度与发育早期的前体细胞无明显区别，提示成体腹腔节小强荧光细胞可能是未分化的前体细胞。

We examined twenty coeliac ganglia of human fetus and newborn, whose embryonic ages ranged from 14 to 40 weeks.The result that early in development, about 14th-16th weeks of gestation, the coeliac ganglia are composed of numerous small intensely catecholamine fluorescent precursots,which formed a condensed group. During later prenatal development,the small intensely fluorescent precursors were gradually replaced by larger principal neurons with increase of embryonic age, which exhibit low intensity fluorescence. When up to parturition, most of cells in the ganglia were principal neurons with a few SIF cells. There are three forms of distribution of SIF cells in human fetal coeliac ganglia, i. e. isolated cells,clusters and small ganglia. These precursors in the ganglia of the early stage of development appear very similar to the SIF cells in the newborn ganglion in size, shape and intensity of fluorescent. This result suggests that the SIS cells in the newborn may be the undifferentiated precursors.