单纯疱疹病毒Ⅰ型SM_（44）株和17Syn~＋株用作跨神经元追踪示踪物的实验研究

A STUDY ON THE USE OF SM_(44) STRAIN AND 17Syn￣+ STRAIN HERPES SIMPLEX VIRUS TYPE 1 AS TRANSNEURONAL TRACERS

单纯疱疹病毒Ⅰ型已被证明是一个很有前途的跨神经元追踪示踪物。它可被轴突顺行或／和逆行运输，还可被跨神经元传递；病毒的株不同，其运输方向亦可不同。本文研究了此病毒ＳＭ４４株和１７ｙｎ＋株在鼠脑内跨神经元传递的方向性和可靠性。结果提示：此两株病毒均能被跨神经元传递，并且以顺行跨神经元传递为主，因而可被作为示踪物用于跨神经元追踪研究。

t bas been proved that Herpes simplex virus type 1 (HSV1) is a useful transneuronal tracer. HSV1 can be transferred transneuronally in the anterograde and/or retrograde direction.The transneuronal transport of the different strains of HSV1 may be in different direritons. It is not known in what direction the SM44 strain and 17Syn+ strain of HSV1 may be transported. In this study, the two strain viruses were injected respectively into the somatomotosensory cortex of the rat brain. After survival times of 4 or 6 days, the sections of the rat brain were processed immnunohistochemically using a monoclonal antibody against HSV1 and ABC method to demonstrate viral antigen. Four days after injection,HSV1-positive neurons were found at sites known to receive input from the cortical neurons within the injection areas,e. g. pontine nucleus, pontine and medullary reticular nuclei,principal sensory trigeminal nucleus, oral part of spinal trigeminal nucleus and inferior olivary nucleus.In addition, "third order" neurons were labelled in the cerebellar cortex,which were known to have no direct fibral connections with the injection areas but receive input from the above nuclei,and in vestibular nucleus, ventral pallidum.These results suggest that both SM44 and 17Syn+ strains of HSV1 can be transneuronally transported mainly in the anterograde direction.Thus,they can be used as an effective tool in the analysis of neuronal circuits.