GABA对大鼠垂体前叶细胞离体增殖的影响及其A受体亚单位mRNA的表达

ACTIONS OF GABA ON THE PROLIFERATION OF CULTURED RAT ANTERIOR PITUITARY CELLS WITH REFERENCE TO IT’S TYPE A RECEPTOR SUBUNIT mRNA EXPRESSION

本实验利用原代细胞培养体系，观察到ＧＡＢＡ对大鼠垂体前叶细胞的氚标胸苷（３Ｈ－ＴｄＲ）基础掺入率有明显的抑制性效应，且可被印防己毒素所反转。培养细胞的原位杂交实验表明，ＧＡＢＡ_Ａ受体的β_２亚单位ｍＲＮＡ可表达于多数垂体前叶细胞中，提示ＧＡＤＡ的上述作用可能系ＧＡＢＡ_Ａ型受体介导的。

This paper describes an inhibitory effert of GABA on the incorporation of￣3H-TdR into rat anterior pituitary cells in static primary monolayer cultures. The action was antagonizable by picrotoxin. In situ hybridization histochemiarry of cultured cells revealed a predominant expression of mRNA encoding β_2 subunit of GABA_A receptor in most of rat anterior pituitary cells, thus suggesting a possible mediation of the above effert by GABA_A receptors.