血小板活化因子刺激肾小球系膜细胞的自分泌作用

The autocrine of cultured glomerular mesangial cells stimulated with PAF in vitro

观察了血小板活化因子（ＰＡＦ）对肾小球系膜细胞（ＧＭＣ）合成ＰＡＦ的影响，结果显示ＰＡＦ能促使ＯＭＣ释放ＰＡＦ，且呈剂量效应依赖关系；ＰＡＦ特异性受体拮抗剂ＢＮ５２０２１（ＢＮ）可明显抑制ＰＡＦ刺激ＧＭＣ产生ＰＡＦ的能力。提示ＰＡＦ可活化ＧＭＣ产生更多的ＰＡＦ，形成一个自分泌放大环路，这在肾组织损伤中可能起着至关重要的作用。ＢＮ可明显地抑制这一作用。因此，本研究为ＰＡＦ拮抗剂治疗肾脏疾病提供了理论依据。

he effect of platelet-activating factor(PAF)on PAF producted by glomerular mesangial cells(CMC )was investigated, Not only PAF could promote the release of PAF by GMC,but also the results showed a dependence relation between dose and response.BN52021(PAF an antagonist ) markedly inhibited the producton of PAF from GMC after PAF stimulation, The findings indicated that PAF could induce GMC to synthesize more PAF,form an enlargement ring of autocrine. With this effect PAF might play an important role in renal tissue injure.The results also subetantiated that BN52021 could markedly inhibit the effect of PAF,It provided theory evidence for PAF antagonist in treatinent of kidney diseases.