海马内移植颈上神经节组织改善穹窿－海马伞横切大鼠的记忆功能

IMPROVEMENT OF MEMORY FUNCTION OF FORNIX-FIMBRIA TRANSECTED RATS BY TRANSPLANTATION OF THE SUPERIOR CERVICAL GANGLION INTO HIPPOCAMPUS

３８只Ｗｉｓｔａｒ大鼠双侧穹窿－海马伞损伤后．以被动回避反应为指标观察到记忆明显受损，与损伤前相比，有记忆动物自６５．３％降至１３．６％，两者差别非常显著。于穹窿－海马伞损伤后记忆丧失动物（ｎ＝１５）自体一侧分离出颈上神经节（ＳＣＧ），切为２－３块并在室温下孵育于２０—５０μｇ／ｍｌ２．５ｓＮＧＦ中１—２ｈ，而后移植于自体双侧海马背侧，移植４周后观察到动物记忆明显恢复，恢复记忆的动物数占７３．３％。在行为实验基础上应用荧光组化方法检查了移植细胞成活情况并测量了海马内去甲肾上腺素（ＮＡ）的含量。移植后２周海马内ＮＡ含量比损伤组有明显上升。移植后一个月，可见部分移植细胞成活并有神经纤维生长。实验表明．穹窿－海马伞损伤大鼠海马内自体移植ＳＣＧ，通过神经递质的局部补充对动物丧失的记忆能力具有一定改善作用。

Memory impairments of passive avoidance response were observed in 38 Wistar rats with bilateral fornix-fimbria transection. After fornix-fimbria lesions the degree of performance decreased from 65. 3 % to 13. 6%(P<0. 01). Autotransplantation of superior cervical ganglion(SCG) into bilateral dorsal hippocampi improved memory function to a considerable extent. In the end of the behavioral experiments, implanted rats were sacrificed for histofluorescence study of grafts and measurement of norepinephrine(NA) content in the hippocampus. These experiments showed that the hippocampul NA content in implanted rats was considerrably higher than that in untrnsplanted fornix-fimbria transected rats and consequently suggested that improvement of memory function was to some extent due to supplement of monoamine transmitter by the transplanted SCG.