测定细胞内自由钙的显微荧光光度计的研制和应用

CONSTRUCTION OF MICROFLUOROMETER AND ITS APPLICATION FOR MEASURING INTRACELLULAR FREE CALCIUM

以ＸＳＪ－２型荧光显微镜为基础，建立了测定细胞内自由钙的显微荧光光度计。为建立这套装置，对荧光显微镜主要作了如下改动：首先，为提供稳定的激发光源，交流汞灯被换成直流流灯；其次，光路中安装了镶有两块干涉滤色片的激发滤色片转盘，使生物样品交替地被两种不同波长的光激发。整套装置的运转在微机控制下。在这套装置上，成功地用Ｆｕｒａ－２测定了细胞内自由钙变化，文中提供了应用实例。

A microfluorumeter used for measuring intracellular free calcium has been constructed.This system was based on an XSJ-2 epifluorescence microscope with the following modifications.First,the AC mercury lamp was replaced by a DC xenon lampto provide a stable light source.Second,,n excitation filter wheel incorporating twointerference filters was inserted in the light path allowing the biological sample to be excited alternately by lights of two different wavelength.The running of the whole system was under the control of a microcomputer. Such a system has successfully beenused to monitor intracellular calcium changes with fluorescent calcium-sensitive dyeFura-2.Some examples of application are presented.